What is the Advantage and Disadvantage of L-Theanine for Nootropic

24 Mar.,2025

 

L-Theanine: Dosage, Benefits, and Side Effects - Healthline

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Theanine benefits, dosage, and side effects - Examine.com

Theanine is most often used for Mental Health. The Examine Database covers Cognitive Improvement, Anxiety, and 15 other conditions and goals.

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Theanine

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Last Updated: September 27,

Theanine, also commonly referred to as L-theanine, is a naturally occurring amino acid found in tea. Supplementation with L-theanine may help reduce stress and anxiety and might improve sleep quality and some aspects of cognitive function.

Theanine is most often used for Mental Health. The Examine Database covers Cognitive Improvement, Anxiety, and 15 other conditions and goals.

Last Updated:September 27,

What is L-theanine?

L-theanine is a naturally occurring amino acid, although it isn't used as a building block for protein synthesis,[1][2] unlike the 20 standard protein-forming amino acids that are synthesized by the genetic code. Several types of tea, including black, oolong, and green tea, contain theanine (generally <50 mg per serving).[3][2][4] When higher doses are desired, theanine can also be taken as a dietary supplement; the theanine in supplements may be extracted from tea[3] or chemically synthesized using bacterial enzymes.[5]

What are L-theanine's main benefits?

The current evidence shows that supplementation with L-theanine may help reduce stress and anxiety in people experiencing acutely stressful situations.[6] Furthermore, L-theanine may also prevent the increase in blood pressure caused by stressful mental tasks.[7] Some small studies have also found that L-theanine might lower depression and anxiety scores in healthy people and in people with major depressive disorder.[8][9]

Supplementation with L-theanine may also improve some aspects of cognitive function, including attention, executive function, and memory.[10][11][8][9] That said, L-theanine has been found to impair executive function and lower attentional control when people are in an emotionally aroused state[12] or are undertaking stressful mental tasks.[13]

Further evidence suggests that supplementation with L-theanine might also enhance sleep quality[8][9] by promoting a more relaxed state in the brain.[14] However, it is important to note that this evidence is derived predominantly from studies of healthy people. There is currently insufficient evidence to make firm conclusions about whether theanine can improve stress, anxiety, depression, cognitive function, or sleep quality in people with chronic conditions.

What are L-theanine's main drawbacks?

The main drawback is that the specific effects of L-theanine have only been tested in a small number of randomized controlled trials which predominantly included healthy people without chronic conditions. Therefore, further high-quality studies are needed to bolster the current evidence.

The clinical evidence cited above shows that supplementation with L-theanine is well-tolerated, with no known reports of adverse effects or toxicity. In rodents, L-theanine has remarkably low toxicity, with one study failing to find any toxic effects when administering up to 4,000 mg of L-theanine per kg of body weight daily for 13 weeks.[15] Additional animal studies have reported similar results with no observed toxicity at remarkably high doses.[1] Consequently, it is generally assumed that L-theanine is safe for humans to consume,[1] particularly given its long history of consumption in tea. However, specific safety studies in humans are lacking.

How does L-theanine work?

After consumption, L-theanine can cross the blood-brain barrier[16] and affect brain activity by promoting increased alpha-wave activity,[17][1][2] a pattern of brain activity associated with a more relaxed state. This may explain its stress-reducing and anxiety-reducing effects.[8]

In animal studies, L-theanine has been shown to affect neurotransmitter signaling in the brain by behaving like a glutamate reuptake inhibitor as well as a glutamate receptor antagonist.[18] Other animal studies demonstrate that L-theanine can also exert neuroprotective effects via its action on gamma-aminobutyric-acid-A (GABA-A) receptors.[19] However, further investigation in humans is needed to confirm these mechanisms of action.[1][2]

What are other names for Theanine ?

Note that Theanine is also known as:

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  • 5-N-Ethyl-Glutamine
  • γ-glutamylethylamide

Research FeedRead all studies

Do Lactium and L-theanine improve sleep?

In this randomized controlled trial, supplementation with a combination of Lactium (a milk protein hydrolysate product) and L-theanine before bed did not reduce insomnia among adults with poor sleep.

Does L-theanine reduce stress?Combined caffeine and theanine supplementation for curling performanceFormulations containing cannabinoids, L-theanine, and gamma-aminobutyric acid for sleepDoes L-theanine enhance cognition?

Frequently asked questions

What is L-theanine?

L-theanine is a naturally occurring amino acid, although it isn't used as a building block for protein synthesis,[1][2] unlike the 20 standard protein-forming amino acids that are synthesized by the genetic code. Several types of tea, including black, oolong, and green tea, contain theanine (generally <50 mg per serving).[3][2][4] When higher doses are desired, theanine can also be taken as a dietary supplement; the theanine in supplements may be extracted from tea[3] or chemically synthesized using bacterial enzymes.[5]

What are L-theanine's main benefits?

The current evidence shows that supplementation with L-theanine may help reduce stress and anxiety in people experiencing acutely stressful situations.[6] Furthermore, L-theanine may also prevent the increase in blood pressure caused by stressful mental tasks.[7] Some small studies have also found that L-theanine might lower depression and anxiety scores in healthy people and in people with major depressive disorder.[8][9]

Supplementation with L-theanine may also improve some aspects of cognitive function, including attention, executive function, and memory.[10][11][8][9] That said, L-theanine has been found to impair executive function and lower attentional control when people are in an emotionally aroused state[12] or are undertaking stressful mental tasks.[13]

Further evidence suggests that supplementation with L-theanine might also enhance sleep quality[8][9] by promoting a more relaxed state in the brain.[14] However, it is important to note that this evidence is derived predominantly from studies of healthy people. There is currently insufficient evidence to make firm conclusions about whether theanine can improve stress, anxiety, depression, cognitive function, or sleep quality in people with chronic conditions.

What is the effect of combining L-theanine with caffeine?

As described above, theanine generally has calming or relaxing effects.[6][7][8][9] On the other hand, caffeine is a powerful central nervous system stimulant that is added to some energy drinks and naturally found in tea, coffee, and chocolate. Because tea contains both L-theanine and caffeine, some clinical studies have investigated the effects of theanine and caffeine individually and in combination. The current evidence shows that theanine might diminish some of the less desirable physiological effects of caffeine. For example, L-theanine has been shown to blunt both caffeine's blood-pressure-increasing effects[20] and its sleep-disrupting effects.[21] However, theanine does not appear to blunt the beneficial effects of caffeine on cognitive performance. Instead, the above-described beneficial effects of theanine on some aspects of cognitive function,[10][11][8][9] such as attention, have been found to be enhanced when caffeine is simultaneously ingested.[22][23][24][25][13] That said, not all studies have found this synergistic effect.[26][20] Overall, dose-response studies of L-theanine and caffeine are currently lacking, and the existing studies are small; larger randomized controlled trials are needed to clarify the interaction between theanine and caffeine. Furthermore, the influence of theanine on the exercise-related performance-enhancing effect of caffeine is currently unclear.

What are L-theanine's main drawbacks?

The main drawback is that the specific effects of L-theanine have only been tested in a small number of randomized controlled trials which predominantly included healthy people without chronic conditions. Therefore, further high-quality studies are needed to bolster the current evidence.

The clinical evidence cited above shows that supplementation with L-theanine is well-tolerated, with no known reports of adverse effects or toxicity. In rodents, L-theanine has remarkably low toxicity, with one study failing to find any toxic effects when administering up to 4,000 mg of L-theanine per kg of body weight daily for 13 weeks.[15] Additional animal studies have reported similar results with no observed toxicity at remarkably high doses.[1] Consequently, it is generally assumed that L-theanine is safe for humans to consume,[1] particularly given its long history of consumption in tea. However, specific safety studies in humans are lacking.

How does L-theanine work?

After consumption, L-theanine can cross the blood-brain barrier[16] and affect brain activity by promoting increased alpha-wave activity,[17][1][2] a pattern of brain activity associated with a more relaxed state. This may explain its stress-reducing and anxiety-reducing effects.[8]

In animal studies, L-theanine has been shown to affect neurotransmitter signaling in the brain by behaving like a glutamate reuptake inhibitor as well as a glutamate receptor antagonist.[18] Other animal studies demonstrate that L-theanine can also exert neuroprotective effects via its action on gamma-aminobutyric-acid-A (GABA-A) receptors.[19] However, further investigation in humans is needed to confirm these mechanisms of action.[1][2]

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