Thermo Fisher Scientific has more than 45 years of experience developing enzymes, buffers, nucleotides, ladders, nucleic acids, and other reagents for life science research. Our dedicated OEM teams partner with customers with product development, manufacturing, and commercialization.
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HM-Taq DNA Polymerase adopts the latest synthetic affinity ligand technology, which can reversibly block the activity of the Taq DNA Polymerase in a temperature-dependent manner. The difference between the HM-Taq DNA Polymerase and the general hot start Taq DNA Polymerase is that the general hot start Taq DNA Polymerase only blocks the polymerase activity before the temperature rise in the first step, while the HM-Taq DNA Polymerase uses the inhibitory ligand to block the substrate-binding site of the HM-Taq DNA Polymerase through temperature regulation. When the temperature is lower than 40°C, an inactive enzyme-inhibitor complex is formed. When the temperature rises to the annealing temperature, the binding balance moves towards the formation of the template-primer complex, so the production of non-specific amplification products in the whole process of PCR amplification is minimized and the accuracy of PCR reaction is greatly improved. The 3' end of PCR product is A, which can be cloned directly with the TA vector.
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